Coding
Part:BBa_K4608005:Design
Designed by: Albert Nitu Group: iGEM23_McGill (2023-10-12)
Truncated Csx30 (trCsx30)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Design considerations were how to best insert this truncated Csx30 protein into GSDMD and GSDME without disrupting the folding of the native gasdermin protein it was inserted into.
Source
Strecker et al., Science 2022 showed that the Csx30 protein which originates from the marine bacterium Desulfonema ishimotonii, coudl be truncated to amino acids 396 to 565 and still be cleaved by its native protease, Csx29.